This test determines the presence of cytochrome oxidase enzymes. penneri (formerly P. vulgaris biogroup 1) was recognized as a new species in 1982 but its role John Lucci 3/25/2014. A methyl red and casein test was done to determine which Gram positive bacteria was and set aside to incubate. After the test was done, the result positive after the addition of the reagent. Unknown Lab Report - PHDessay.com Microbiology: Unknown Organism Lab Report Example | Topics ... Proteus vulgaris - Wikipedia If time had permitted, an Indole and Simmon's Citrate test would have been conducted to prove the bacteria of the unknown. in a 2D pattern while the rest of tube is clear like A, the uninoculated tube. It's notably useful for Salmonella, Escherichia coli Francisella, Citrobacter freundii, Salmonella species, Proteus mirabilis, Proteus vulgaris, Edwardsiella tarda, and Proteus species identification. The genus Proteus currently consists of five named species (P. mirabilis, P. penneri, P. vulgaris, P. myxofaciens, and P. hauseri) and three unnamed genomospecies (Proteus genomospecies 4, 5, and 6).. The literature abounds with studies on the taxonomy of the genus Proteus since the original publication by Hauser, who first described the genus (Table (Table1) 1) (). Proteus Vulgaris is a pole molded, nitrate-lessening, indole+ and catalase-positive, hydrogen sulfide-delivering, Gram-negative bacterium that possesses the intestinal tracts of people and creatures. medium: The SIM medium is a multitest agar used to test for indole production while simultaneously determining motility and hydrogen sulfide producing abilities of the . Biochemical Test of Enterobacter aerogenes. S production on Kligler Agar (Prod No 60787) but not on Triple Sugar Iron Agar. Use only plastic or platinum loops for this test. Through a vast series of test result complications, our groups unknown bacterium was. Consider a slant, inject Simmons citrate agar gently on the slant by slightly touching the tip of the needle to the colony, which is 18 - 24 hours old. S. first and foremost the most identifications of test for proteus spp by urease test ,PPA The socond biochemical test indole the are defrenciate to p. Mirabilis from p vulgaris. The genus Streptococcus is a complex group causing a wide range of diseases such as: rheumatic fever, impetigo, pharyngitis, laryngitis, toxic shock syndrome, scarlet fever, and endocarditis. Biochemical Test Chart (You will not fill in every blank in this chart.) Escherichia coli, Proteus vulgaris, and Shigella flexneri. Introduction of H2S Positive Bacteria. VULGARIS) Shape - Proteus Vulgaris is a short, straight rod shape (bacillus) bacterium.. S positive on Triple Sugar Iron Agar. If an organism is motile than the growth will radiate from the stab mark and make the entire tube appear turbid. Both P. vulgaris and P. mirabilis are VP negative. The SIM medium for the H 2 S production test was prepared according to past literature. Image Source: MicrobeWiki and Getty Images. The next method used was a Urea test. H 2 S positive bacteria have the ability to produce hydrogen sulfide from substrates such as sulfur-containing amino acids or inorganic sulfur compounds. The use of an iron-containing metal inoculation loop can lead to a false-positive reaction. P. vulgaris produces an acid butt, an acid or alkaline slant, H2S, and gas. If an organism is motile than the growth will radiate from the stab mark and make the entire tube appear turbid. The LDC and ODC test results for three bacterial species of the ATCC strain could be determined 4 hours after inoculation . These Proteus strains are used in an agglutination test (the Weil-Felix test) for serum antibodies produced against rickettsiae of the typhus and spotted fever groups. An even easier way to test this is the gelatinase test. Proteus vulgaris is variable, it maybe positive or negative. E. coli, Proteus mirabilis, K. pneumoniae, K. oxytoca, S, dysentry, E. aerogens. Something to think about… • Look at the results for the tests in the file again, do you notice some type of pattern? Using the results below and the attached differential tests chart only, determine which single test you can do that would tell you with a high level of certainty which organism you have. It is grouped with the Morganellaceae and is an opportunistic pathogen of humans. The test is performed on Simmons citrate agar: Negative citrate utilization test is indicated by the lack of growth and color change in the tube. Identifying an unknown - S. flexneri and P. vulgaris with lactose fermentation? A contamination must have happened running these tests because the results were never accurate. Cultures:- Nutrient broth cultures of Micrococcus luteus and Proteus vulgaris (young cultures) Procedure: Wet Mount Preparation . Animal peptones and yeast extract provide the nutritive base. Record the results. 5) Answer the questions below referring to the SIM test and Kligler's Iron Agar test, respectively. The indole test is a component of the IMViC series of tests, which is used for differentiating the Enterobacteriaceae. Indole Test How to Perform Test: Inoculate Tryptone broth with inoculating loop. Proteus Vulgaris . P. vulgaris possesses peritrichous flagella, making it actively motile. With further testing by spot indole, the positive isolates may be presumptively reported as Proteus vulgaris and the negative ones as Proteus mirabilis. Point to remember: Indole test can also aid in species differentiation. Sulfur-indole-motility test results from various microbes. Production of indole from tryptophan is an important biochemical property of Escherichia coli, many strains of group A, B, and C Shigella, Edwardsiella tarda, Klebsiella oxytoca, and Proteus vulgaris. Pseudomonas aeruginosa and the strain of Proteus mirabilis that we work with are motile. Proteus vulgaris is a rod-shaped, nitrate-reducing, indole-positive and catalase-positive, hydrogen sulfide-producing, Gram-negative bacterium that inhabits the intestinal tracts of humans and animals. I designed this video from my Text books not. as nitrate can interfere with the spot indole test by inducing false-negative results. Alcaligenes faecalis Bacillus megaterium Bacillus subtilis Enterobacter aerogenes Enterobacter cloacae Enterococcus faecalis Escherichia coli Klebsiella pneumoniae Micrococcus luteus Micrococcus roseus Proteus mirabilis Proteus vulgaris Pseudomonas aeruginosa Indole: Positive. After accurately conducted test, the results of Nitrate test and MR-VP tests . moniae (Kp) Bacillus . Some members of the Enterobacteriaceae and H. 2. the enzyme cysteine desulferase catalyzes putrefication of the amino acid cysteine into pyruvate . These results were indicated by a red color change at the top of the tube that indicated Indole production, black coloring that indicated sulfur reduction, and a radiation of growth from the stab mark, which indicated motility. Proteus; Description: It is a genus of Gram-negative bacteria which belongs to family Enterobacteriaceae.They can be isloated from sewage,soil,human and animal feces.Some of its species are human pathogen including Proteus vulgaris, Proteus mirabilis, and Proteus Penneri.They are opportunistic pathogens which can cause wound and urinery tract . Specifically, the urea test was positive, the H 2 S test was positive, and the indole test was positive. For Part C, label Staphylococcus epidermis, Proteus vulgaris, and Escherichia coli on the Petri plate with the MacConkey agar. You are performing a single unknown and have performed the SIM test and Lysine Decarboxylase test on your gram negative organism. Sulfide and Motility Test Using SIM media. Results: 11-15-14 Gram - is hydrolase positive, gram + is hydrolase negative. The second unknown was a gram negative rod and that bacterium had three separate tests performed. Record your observations & interpretations of . Proteus vulgaris (Pv) Klebsiella pneu. Ironically, a test that would confirm P. vilgaris, urease production, was not partaken, but the positive indicators of sulfur reduction, tryptophan deaminase production, indole production, gelatinase activity and saccharose fermentation, are strong indicators of P. vilgaris colonies.8 Proteus vulgaris and Proteus penneri are easily isolated . Sulfur reduction test, Indole production, Motility (SIM) deeps all 3 tests done w/SIM deeps just add Kovac's reagent for Indole test • Alcaligenes faecalis (left) - • Escherichia coli (middle) - • Proteus vulgaris (black precipitate) + • Reagent: Ferrous ammonium sulfate-indicator. Using aseptic technique, inoculate the labeled parts of the plate. Tube B has growth along the stab line . Lactose negative organisms: Lactose positive organisms: SIM medium: Citrate medium: Oxidase Test: Urease medium: Pseudomonas aeruginosa SIM (-), Oxidase (+) Escherichia coli Enzymatic Reactions. A urea test, testing for the activated enzyme urease was positive, ruling out E. coli. The plate was then incubated at 37 degree's C for 8 hrs, culture was young to image the start of swarming. Kovac's reagent was added following the incubation period. It can be found in soil, water, and fecal matter. vulgaris is arranged singly, in pairs, or in short chains and sometimes in clusters.. MORPHOLOGY OF PROTEUS VULGARIS (MICROSCOPIC VIEW) TOP The motility test is useful for testing a wide variety of organisms. After this final test was performed, enough information was obtained to confirm the identity of the unknown Gram-negative bacterium . The left image depicts a positive hydrogen sulfide reaction as well as motility of the test organism (Salmonella enterica serovar Typhimurium). Tube A is uninoculated. A negative VP result indicated the bacterium did not produce acetyl methyl carbinol and could only be Escherichia coli or P. vulgaris. Pseudomonas aeruginosa and the strain of Proteus mirabilis that we work with are motile. After incubation the broth was still a yellow color, giving a negative result. The SIM test showed positive for Sulfur, Indole, and motility. Found in soil, water, and fecal matter. A positive result is a red color in the liquid, and a negative result would be a yellowish color. Lysine Decarboxylase Test. Proteus vulgaris is variable, it maybe positive or negative. Determinative Bacteriology of Proteus vulgaris. Proteus species: Proteus vulgaris is indole positive whereas Proteus mirabilis is . The following shows results of three bacterial strains, Edwardsiella tarda, Klebsiella pneumoniae, and Proteus vulgaris, grown on SIM. SIM medium contains nutrients, iron, and sodium thiosulfate. CAMP Test- Principle, Procedure, Types, Results, Uses, Limitations Categories Biochemical Test of Bacteria Tags Proteus , Proteus mirabilis Post navigation Biochemical Test of Streptococcus pneumoniae SIM test: Sulfur Indole Motility test is for sulfide production and motility which is indicated by the formation of cherry red color in indole test and bacterial spread in media indicates motile organism. Gram-negative; Non-spore-forming rods This can happen because of sucrose in the medium, which suppresses The positive indole result given by Escherichia coli (right) is indicated by the red layer at the top of the tube after the addition of Kov. The H 2 S formed reacts with the iron salt ammonium iron (III) citrate , which is also contained in the medium, to form iron sulfide, which is precipitated as a black . After addition of Kovács reagent, a pink ring at the top of the tube indicates a positive indole result (A and E). The result can be alpha, beta, or gamma hemolysis. All three are facultatively anaerobic gram-negative rods. From left to right: (A) Escherichia coli, (B) Staphylococcus aureus, (C) Salmonella arizonae, (D) Enterobacter aerogenes, and (E) Proteus vulgaris. Determinative Bacteriology of Proteus vulgaris. Results: 11-15-14 Gram - is hydrolase positive, gram + is hydrolase negative. It is known to cause wound infections and other species of its . If an organism is motile than the growth will radiate from the stab mark and make the entire tube appear turbid. The presence of the bile salts and dyes inhibit most gram-positive organisms allowing only gram-negative rods to grow on HE agar. narrowed the possible bacterium down to three with a Vogues-Proskauer test following. The . The genus Proteus is classified in the enteric bacteria, together with Escherichia coli, Salmonella, Shigella, Enterobacter and Serratia.All these bacteria are small, Gram-negative rods and are facultative anaerobes: they ferment sugars in anaerobic conditions but can use a wide range of organic molecules in aerobic conditions. Size - The size of Proteus Vulgaris is about 1-3 µm × 0.5 µm (micrometer).. iv. This test consisted of stirring the bacterial growth into a tube of phenol red and urea to test for the presence of acid. After the results from this test were read and recorded, the final test, the SIM agar test was performed by inoculating the Gram-negative colony into a test tube and incubating for 48 hours at 37 degrees Celsius. Hektoen enteric agar is a selective and differential media for the isolation and differentiation of enteric pathogens from clinical specimens. The SIM culture medium contains the inorganic compound sodium thiosulphate.Some representatives of the Enterobacteriaceae, such as B. Salmonella and Proteus are able to reduce such sulfur compounds to hydrogen sulfide (H 2 S) . Red coloration indicates the positive results. The bacteria that are able to completely lyse RBC will show visible clearing of the agar and is positive for beta hemolysis. - Proteus vulgaris - Shigella flexneri. RESULTS: For the Gram negative rods, the indole test came back positive which left me between Escherichia coli or Proteus vulgaris. The size of the individual cells varies from 0.4 to 0.6 micrometers by 1.2 to 2.5 micrometers. In a TSI tube, E. coli produces an acid butt, an acid or alkaline slant, and no H2S, but does produce gas.